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Product Info Summary
| SKU: | LS008233 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of zinc finger (CCCH type), RNA-binding motif and serine/arginine rich 2 (ZRSR2) |
Product info
Product Name
ZRSR2 (NM_005089) Human Over-expression Lysate
SKU/Catalog Number
LS008233
Size
100 µg
Description
Transient overexpression lysate of zinc finger (CCCH type), RNA-binding motif and serine/arginine rich 2 (ZRSR2)
Storage & Handling
Cite This Product
ZRSR2 (NM_005089) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008233)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ZRSR2 Human Over-expression Lysates NM_005089.
Western blot validation of overexpression lysate (LS008233) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZRSR2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ZRSR2 (Source: Uniprot.org, NCBI)
Gene Name
ZRSR2
Full Name
U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
Weight
58.045kDa
Alternative Names
CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2; Renal carcinoma antigen NY-REN-20; U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-relatedprotein 2; U2 small nuclear ribonucleoprotein auxiliary factor, small subunit 2; U2(RNU2) small nuclear RNA auxiliary factor 1-like 2MGC142014; U2AF1L2; U2AF1RS2MGC142040; U2AF1-RS2U2AF35-related protein; URPU2 small nuclear RNA auxiliary factor 1-like 2; zinc finger (CCCH type), RNA-binding motif and serine/arginine rich 2 ZRSR2 U2AF1-RS2, U2AF1L2, U2AF1RS2, URP, ZC3H22 zinc finger CCCH-type, RNA binding motif and serine/arginine rich 2 U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2|U2 small nuclear ribonucleoprotein auxiliary factor, small subunit 2|U2(RNU2) small nuclear RNA auxiliary factor 1-like 2|U2AF35-related protein|renal carcinoma antigen NY-REN-20|zinc finger (CCCH type), RNA binding motif and serine/arginine rich 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ZRSR2, check out the ZRSR2 Infographic
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In this infographic, you will see the following information for ZRSR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ZRSR2 (NM_005089) Human Over-expression Lysate (LS008233)
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